identification of phid gene with fluorescent pseudomonads from rhizospheric zone of chickpea and its relation with biological control of chickpea fusarium wilt disease caused by fusarium oxysporum f. sp. ciceris
نویسندگان
چکیده
fusarium wilt of chickpea, caused by f. oxysporum f. sp. ciceris, is one of the most important diseases of this plant in iran. in order to control this disease, fluorescent pseudomonads were isolated from the rhizosphere of chickpea plants in khorasan province by using king,s b medium (kb). antifungal activity of 80 bacterial strains against f. oxysporum f. sp. ciceris was evaluated on kb and potato dextrose agar (pda) media. results revealed that from 80 strains tested, 81.25% of in kb and 94.37% in pda had the ability to inhibit fungal growth. there was a correlation between production antifungal metabolites and biocontrol ability of strains, but there was not any significant correlation between sidrophors production and biocontrol ability the strains. detection of phld, which is considered as the key gene for production of 2, 4- diacetylphloroglucinol biosynthesis in bacterial strains was performed using polymerase chain reaction technique (pcr) and specific primers b2bf/bpr4. results indicated that 20 strains were phld+ and a specific band of 629 bp was amplified in these strains. the results of experiments realized under greenhouse conditions, showed that only m2-15 isolate reduced significantly fusarium wilt in chickpea, with the rest having positive effect on chickpea growth factors. t26, m2-15 and t90 isolates caused a significant increase in growth factors including dry and fresh root and shoot weights comparing to those of control plants. among strains studied in this research, m2-15 significantly decreased chickpea wilt under greenhouse conditions.
منابع مشابه
Temperature Response of Chickpea Cultivars to Races of Fusarium oxysporum f. sp. ciceris, Causal Agent of Fusarium Wilt
Blanca B. Landa, College of Agriculture (ETSIAM), University of Córdoba (UCO), P.O. Box 3048, 14080 Córdoba, Spain; Juan A. Navas-Cortés, Institute of Sustainable Agriculture (IAS), Spanish Council for Scientific Research (CSIC), P.O. Box 4084, 14080 Córdoba, Spain; María del Mar Jiménez-Gasco, Department of Plant Pathology, The Pennsylvania State University, University Park, PA 16802, USA; Jaa...
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ABSTRACT Fusarium oxysporum f. sp. ciceris and the root-lesion nematode Pratylenchus thornei coinfect chickpeas in southern Spain. The influence of root infection by P. thornei on the reaction of Fusarium wilt-susceptible (CPS 1 and PV 61) and wilt-resistant (UC 27) chickpea cultivars to F. oxysporum f. sp. ciceris race 5 was investigated under controlled and field conditions. Severity of Fusar...
متن کاملIn planta and soil quantification of Fusarium oxysporum f. sp. ciceris and evaluation of Fusarium wilt resistance in chickpea with a newly developed quantitative polymerase chain reaction assay.
Fusarium wilt of chickpea caused by Fusarium oxysporum f. sp. ciceris can be managed by risk assessment and use of resistant cultivars. A reliable method for the detection and quantification of F. oxysporum f. sp. ciceris in soil and chickpea tissues would contribute much to implementation of those disease management strategies. In this study, we developed a real-time quantitative polymerase ch...
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Fusarium oxysporum f. sp. ciceri (Foc), one of the most important fungal pathogen of chickpea, is a constant threat to this crop plant. In the present study gene expression analysis of chickpea roots during Foc infection was performed using various approaches. cDNAs derived from total mRNA during infection process of susceptible (JG62) and resistant (Digvijay) cultivars, were amplified using ra...
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The effects of temperature and inoculum density of Fusarium oxysporum f. sp. ciceris race 5 on suppression of Fusarium wilt in chickpea (Cicer arietinum) cv. PV 61 by seed and soil treatments with rhizobacteria isolated from the chickpea rhizosphere were studied in a model system. Disease development over a range of temperatures (20, 25, and 30 degrees C) and inoculum densities (25 to 1,000 chl...
متن کاملQuantitative Modeling of the Effects of Temperature and Inoculum Density of Fusarium oxysporum f. sp. ciceris Races 0 and 5 on Development of Fusarium Wilt in Chickpea Cultivars.
ABSTRACT Races 0 (Foc-0) and 5 (Foc-5) of Fusarium oxysporum f. sp. ciceris differ in virulence and induce yellowing or wilting syndrome, respectively, in chickpea. We modeled the combined effects of soil temperature and inoculum density of Foc-0 and Foc-5 on disease developed in chickpea cvs. P-2245 and PV-61 differing in susceptibility to those races, using quantitative nonlinear models. Dise...
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عنوان ژورنال:
حفاظت گیاهانجلد ۲۷، شماره ۴، صفحات ۴۰۷-۰
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